Development of positive control assays for the detection of Bacillus anthracis plasmids PXO1 AND PXO2 via PCR
نویسندگان
چکیده
منابع مشابه
Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR.
We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR.
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Involvement of the pagR gene of pXO2 in anthrax pathogenesis
Anthrax is a disease caused by Bacillus anthracis. Specifically, the anthrax toxins and capsules encoded by the pXO1 and pXO2 plasmids, respectively, are the major virulence factors. We previously reported that the pXO1 plasmid was retained in the attenuated strain of B. anthracis vaccine strains even after subculturing at high temperatures. In the present study, we reinvestigate the attenuatio...
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ژورنال
عنوان ژورنال: Bulletin "Veterinary biotechnology"
سال: 2018
ISSN: 2306-9961
DOI: 10.31073/vet_biotech32(1)-3